Evaluation of Two Different Lipopolysaccharide Extraction Methods for Purity and Functionality of LPS

Lipopolysaccharide is a virulent cell membrane component of Gram negative bacteria. It is also a potent activator of immune system capable of triggering cytokine release from cells of different origin. Life threatening diseases as septic shock following infection with Gram negative bacteria is mediated mainly by LPS (Kawaiand Akira, 2010). Lipopolysaccharide (LPS), is a complex structure and is composed of three parts namely, lipid A (immunologic), polysaccharide (role in membrane penetration) and specific O side chain (antigenicity). Lipid A is highly conserved and exerts the endotoxic activity, while the ′′O′′ antigen is a carbohydrate polymer of repeating oligosaccharides, which differs between species and is responsible for the serological specificity of bacteria (Prescott et al., 2002). LPS causes pathophysiological effects such as fever, leucopenia, leucocytosis etc. (Luderitz et al., 1973) which thus focused International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 3 (2017) pp. 1296-1302 Journal homepage: http://www.ijcmas.com